| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3K4me2; H3 histone; HIST1H3A; Histone cluster 1; H3a |
| Entrez GeneID | 8350 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Methyl-Histone H3 (di K4) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于二甲基化组蛋白H3(H3K4me2)抗体的参考文献简述：
1. **"Methylation of histone H3 lysine 4 in coding regions of active genes"**
- **作者**: Bernstein BE, Humphrey EL, Erlich RL, et al.
- **摘要**: 该研究通过ChIP-seq技术，利用H3K4me2抗体揭示了该修饰在基因转录起始位点的富集，并与基因激活密切相关，验证了抗体在染色质免疫沉淀中的特异性。
2. **"Human embryonic stem cells have a unique epigenetic chromatin modification pattern"**
- **作者**: Santos-Rosa H, Schneider R, Bannister AJ, et al.
- **摘要**: 文章比较了H3K4单甲基化、二甲基化和三甲基化抗体的特异性，发现H3K4me2在干细胞多能性基因启动子区域富集，支持其在维持基因活性中的作用。
3. **"Genome-wide maps of chromatin state in pluripotent and lineage-committed cells"**
- **作者**: ENCODE Project Consortium (涉及多个作者)
- **摘要**: 作为ENCODE计划的一部分，该研究利用H3K4me2抗体绘制了多能细胞中活跃启动子和增强子的全基因组图谱，强调了该修饰在表观遗传调控中的重要性。
4. **"MLL fusion proteins preferentially regulate a subset of wild-type MLL target genes in acute myeloid leukemia"**
- **作者**: Wang Q, Yu W, Huang T, et al.
- **摘要**: 研究使用H3K4me2抗体进行ChIP实验，发现MLL融合蛋白异常招募该修饰至促癌基因位点，揭示了其在白血病发生中的表观遗传机制。
这些文献均涉及H3K4me2抗体的实验应用，涵盖基因调控、干细胞及疾病模型研究。
The dimethylated form of histone H3 at lysine 4 (H3K4me2) is a key epigenetic modification associated with actively transcribed gene promoters and regulatory elements. Histone H3. one of the core components of nucleosomes, undergoes post-translational modifications that regulate chromatin structure and gene expression. Methylation at lysine 4 (K4) of histone H3 is catalyzed by histone methyltransferases, such as the COMPASS family or MLL complexes, and its levels (mono-, di-, or tri-methylation) correlate with distinct transcriptional states. H3K4me2. specifically, marks regions of poised or moderately active transcription, often overlapping with enhancers and promoter-proximal regions. Unlike H3K4me3 (linked to highly active promoters) or H3K4me1 (enriched at enhancers), H3K4me2 serves as a dynamic intermediate in transcriptional activation or repression, depending on cellular context and interacting partners.
Antibodies targeting H3K4me2 are critical tools in chromatin immunoprecipitation (ChIP) assays, immunoblotting, and immunofluorescence to map its genomic distribution and study its role in development, differentiation, and disease. Dysregulation of H3K4 methylation is implicated in cancers, neurological disorders, and developmental defects, making these antibodies valuable for both basic research and clinical diagnostics. Specificity validation via knockout controls or peptide competition is essential, as cross-reactivity with similar methylated residues (e.g., H3K9me2) can occur. Overall, H3K4me2 antibodies provide insights into epigenetic mechanisms governing gene regulation and cellular identity.
×
