| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3K18me; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J |
| Entrez GeneID | 8350 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3 (mono methyl K18) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及MonoMethyl-Histone H3 (Lys18)抗体的参考文献，按研究背景概括：
1. **"Site-specific methylation of histone H3K18 promotes oncogenesis through regulation of gene expression programs"**
*Authors: Smith et al.*
摘要：该研究揭示H3K18单甲基化(H3K18me1)在癌症中的关键作用，使用特异性抗体通过ChIP-seq发现其富集于致癌基因启动子区域，促进细胞增殖和肿瘤发生。
2. **"Dynamic regulation of histone H3K18 methylation during cellular differentiation"**
*Authors: Li & Zhang*
摘要：通过免疫荧光和Western blot分析，发现H3K18me1在干细胞分化过程中动态变化，抗体特异性验证表明其与染色质重塑复合物相互作用，调控分化相关基因沉默。
3. **"Antibody specificity profiling for histone post-translational modifications using peptide microarray"**
*Authors: Rothbart et al.*
摘要：该文献系统评估了多种组蛋白修饰抗体的特异性，包括H3K18me1抗体，证实其在不同实验条件(如ChIP、IF)中的可靠性，并排除对相邻位点(如H3K17/K19)的交叉反应。
注：以上文献名为虚构概括，实际研究中H3K18me1相关文献较少，建议结合具体实验通过PubMed或CiteAb(抗体引用数据库)检索实际使用该抗体的论文。
The MonoMethyl-Histone H3 (Lys18) antibody is a specialized tool used to detect monomethylation at lysine 18 on histone H3 (H3K18me1), a post-translational modification involved in chromatin regulation and gene expression. Histone H3 is a core component of nucleosomes, and its tail modifications, including methylation, acetylation, and phosphorylation, play critical roles in epigenetic signaling. Methylation at Lys18 is catalyzed by histone methyltransferases such as SUV420H1/2 and is associated with both transcriptional activation and repression, depending on the cellular context and interacting partners.
H3K18me1 has been implicated in DNA damage response, cell cycle regulation, and maintaining genomic stability. Studies suggest its role in recruiting repair proteins to sites of DNA damage and influencing heterochromatin formation. Dysregulation of H3K18 methylation is linked to cancer progression, developmental disorders, and aging, making it a biomarker of interest in disease research.
This antibody is widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map epigenetic changes, study chromatin dynamics, or investigate disease mechanisms. Specificity is validated through knockout models or peptide competition assays to ensure recognition of the monomethylated Lys18 epitope. Researchers utilize it to explore how H3K18me1 interacts with other histone marks or environmental cues to regulate cellular processes, offering insights into epigenetic therapies and diagnostic strategies.
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