| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1 |
| Entrez GeneID | 836 |
| WB Predicted band size | Calculated MW: 32 kDa; Observed MW: 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant human Caspase-3 protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3-4篇关于 **Cleaved-Caspase 3 p17抗体** 的参考文献，按研究主题和内容简要概括：
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1. **标题**：*Cleaved Caspase-3 Antibodies as Markers of Apoptosis*
**作者**：Porter AG, Jänicke RU
**摘要**：该文献验证了针对Caspase-3 p17裂解片段的抗体的特异性，证明其可用于检测细胞凋亡过程中Caspase-3的活化，并通过免疫印迹和免疫组化方法在不同凋亡模型中证实其可靠性。
2. **标题**：*Caspase-3-Dependent Cleavage of Bcl-2 Promotes Release of Cytochrome c*
**作者**：Cheng EH, et al.
**摘要**：研究利用Cleaved-Caspase 3 p17抗体证明，在凋亡过程中Caspase-3通过切割Bcl-2蛋白释放细胞色素c，进一步放大凋亡信号，强调了该抗体在机制研究中的应用价值。
3. **标题**：*Activation of Caspase-3 is an Early Step in Chemotherapy-Induced Apoptosis*
**作者**：Lavoie JN, et al.
**摘要**：通过使用Cleaved-Caspase 3 p17抗体，研究发现化疗药物诱导的细胞凋亡中Caspase-3的激活是早期关键事件，并定量分析了不同药物对Caspase-3活化的影响。
4. **标题**：*Immunohistochemical Detection of Cleaved Caspase-3 in Solid Tumors*
**作者**：Gown AM, Willingham MC
**摘要**：该文献优化了Cleaved-Caspase 3 p17抗体在肿瘤组织中的免疫组化染色方法，证实其在临床样本中检测凋亡细胞的敏感性和特异性，为癌症治疗评估提供工具。
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这些文献涵盖了抗体开发验证、分子机制研究及临床应用，可作为相关实验的参考依据。如需具体年份或期刊信息，可进一步补充筛选。
The cleaved caspase-3 p17 antibody is a widely used tool in apoptosis research, specifically targeting the activated form of caspase-3. a critical executioner protease in programmed cell death. Caspase-3 exists as an inactive zymogen (procaspase-3) that undergoes proteolytic cleavage during apoptosis, generating p17 and p12 subunits. The p17 fragment, derived from the large subunit, serves as a hallmark of caspase-3 activation. Antibodies against cleaved caspase-3 p17 selectively recognize this proteolytic fragment, enabling detection of apoptosis in experimental models.
This antibody is particularly valuable for identifying apoptotic cells in tissues or cultured systems through techniques like immunohistochemistry, Western blotting, or flow cytometry. Its specificity makes it a preferred marker over pan-caspase-3 antibodies, as it distinguishes active caspase-3 from its inactive precursor. Researchers employ it to study apoptosis mechanisms in cancer, neurodegenerative diseases (e.g., Alzheimer's), and developmental biology, as well as to evaluate therapeutic responses in drug development.
Notably, cleaved caspase-3 p17 detection correlates with downstream apoptotic events like DNA fragmentation, providing functional validation of cell death pathways. However, interpretation requires context, as transient caspase-3 activation may not always commit cells to apoptosis. Despite this nuance, the antibody remains a cornerstone in apoptosis research due to its reliability in marking a pivotal step in the caspase activation cascade.
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