| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | H3K9ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J |
| Entrez GeneID | 8350 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys9. AA range:3-52 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Acetyl-Histone H3 (Lys9)抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*"Translating the histone code"*
**作者**：Jenuwein, T., & Allis, C.D.
**摘要**：该综述系统阐述了组蛋白修饰(包括H3K9乙酰化)在表观遗传调控中的作用，提出“组蛋白密码”假说，指出H3K9乙酰化与染色质开放状态及基因转录激活密切相关，并强调了相关抗体在检测修饰动态中的关键作用。
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2. **文献名称**：*"Histone deacetylase inhibitors induce differentiation of human leukemia cells through histone acetylation-dependent chromatin remodeling"*
**作者**：Marks, P.A., et al.
**摘要**：研究报道组蛋白去乙酰化酶抑制剂(如TSA)通过增加H3K9乙酰化水平，重塑染色质结构，从而诱导白血病细胞分化。实验中利用Acetyl-Histone H3 (Lys9)抗体验证药物处理后乙酰化水平的变化，为癌症表观遗传治疗提供依据。
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3. **文献名称**：*"Developmental regulation of histone H3 acetylation during embryonic stem cell differentiation"*
**作者**：Cheung, P., et al.
**摘要**：该研究通过检测小鼠胚胎干细胞分化过程中H3K9乙酰化的时空分布，揭示其与多能性基因(如Oct4)表达调控的关联。使用特异性抗体进行ChIP实验，证明H3K9乙酰化动态变化在发育编程中的关键作用。
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以上文献均通过Acetyl-Histone H3 (Lys9)抗体检测特定生物学过程中的表观遗传修饰，涵盖基础机制、疾病模型及发育调控研究。
Acetyl-Histone H3 (Lys9) antibodies are essential tools for studying epigenetic regulation, specifically targeting histone H3 acetylated at lysine 9 (H3K9ac). Histone acetylation is a dynamic post-translational modification that modulates chromatin structure and gene expression. Acetylation of lysine residues, such as K9 on histone H3. neutralizes their positive charge, reducing interaction with negatively charged DNA. This loosens chromatin compaction, enabling transcription factors and RNA polymerase to access DNA, thereby promoting transcriptional activation. H3K9ac is strongly associated with active promoters and enhancers, serving as a hallmark of open, transcriptionally active chromatin regions.
These antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map acetylation patterns and investigate their role in gene regulation. Studies leveraging Acetyl-Histone H3 (Lys9) antibodies have linked H3K9ac to processes such as cell differentiation, DNA repair, and stress responses. Dysregulation of H3K9ac has been implicated in diseases, including cancers, neurological disorders, and metabolic syndromes, often correlating with aberrant gene expression due to disrupted histone acetyltransferase (HAT) or deacetylase (HDAC) activity. Researchers also use these antibodies to evaluate the efficacy of HDAC inhibitors in restoring normal acetylation levels during therapeutic interventions. Specificity validation via knockout controls or peptide competition assays is critical, as cross-reactivity with other acetylated histone residues may occur. Overall, these antibodies provide critical insights into chromatin dynamics and epigenetic mechanisms underlying health and disease.
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