Rabbit Monoclonal Cleaved-Caspase3p12 Antibody

The cleaved caspase-3 p12 antibody is a critical tool for detecting activated caspase-3. a central protease in apoptosis. Caspase-3. a member of the cysteine-aspartic acid protease family, exists as an inactive zymogen (procaspase-3) under normal conditions. During apoptosis, upstream initiator caspases (e.g., caspase-8. -9) cleave procaspase-3 at specific aspartic acid residues, generating active fragments: a p17 subunit and a p12 subunit. The cleaved caspase-3 p12 antibody specifically recognizes the 12 kDa fragment (p12) of the activated enzyme, serving as a marker for caspase-3 activation. This antibody is widely used in apoptosis research to confirm programmed cell death in experimental models, including cancer, neurodegeneration, and developmental studies. Its specificity for the cleaved form minimizes cross-reactivity with the inactive precursor, enabling accurate detection of apoptosis in techniques like Western blotting, immunohistochemistry, and flow cytometry. The p12 subunit, though less studied than p17. contributes to the structural stability of the active caspase-3 heterotetramer (p17₂/p12₂). Researchers rely on this antibody to evaluate therapeutic efficacy, drug-induced apoptosis, or disease mechanisms linked to dysregulated cell death. Proper validation with positive/negative controls is essential due to potential non-specific signals in certain cell types or tissues.