首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠组织型纤溶酶原激活剂(t-PA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAT含量。PLAT 即组织型纤溶酶原激活剂，是重要的血栓溶解因子。其背景在于在人体纤溶系统中起关键作用。研究机制主要是能特异性地激活血栓中的纤溶酶原，使之转化为纤溶酶，进而降解纤维蛋白，达到溶解血栓的效果。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于心脑血管疾病机制研究、药物干预效果评价以及凝血与纤溶系统平衡相关的基础科研实验，为探索t-PA在组织损伤修复、血栓形成与溶解等过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Plat ELISA Kit; Tissue-type plasminogen activator ELISA Kit; t-PA ELISA Kit; t-plasminogen activator ELISA Kit; tPA ELISA Kit; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B] ELISA Kit
缩写：PLAT
Uniprot No.：P19637
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of rat t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 96-106 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected201.9461.8461.8961.753101.5421.4401.4911.34851.0190.9891.0040.8612.50.5360.5290.5330.3901.250.3300.3200.3250.1820.6250.2780.2680.2730.1300.3120.2050.2010.2030.06000.1440.1420.143 | ||||||
货期：3-5 working days
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