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产品描述：人钙依赖性磷脂酶A2(PLA2G5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLA2G5含量。PLA2G5是一种重要靶点。它属于磷脂酶A2家族成员，在机体炎症反应、细胞膜代谢等过程中发挥作用。研究机制方面，主要聚焦其活性调节、与其他信号通路的相互作用，解析其在疾病发生发展中的角色以探索潜在治疗策略。试剂盒检测范围为0.18 ng/mL-12 ng/mL，本产品适用于基础科研领域，如探究PLA2G5在动脉粥样硬化、肥胖相关脂代谢异常或炎症性疾病模型中的表达变化，亦可用于评估药物干预对磷脂酶活性调控的体外研究，为脂质信号通路机制解析提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ca2+ dependent phospholipase A2 ELISA Kit; Calcium dependent phospholipase A2 ELISA Kit; Calcium-dependent phospholipase A2 ELISA Kit; DKFZp686C2294 ELISA Kit; FRFB ELISA Kit; Group V phospholipase A2 ELISA Kit; GV PLA2 ELISA Kit; gVPLA2 ELISA Kit; hVPLA(2) ELISA Kit; MGC46205 ELISA Kit; OTTHUMP00000044655 ELISA Kit; PA2G5_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 5 ELISA Kit; Phospholipase A2 group V ELISA Kit; PLA2 10 ELISA Kit; PLA2 G5 ELISA Kit; PLA2-10 ELISA Kit; Pla2g5 ELISA Kit; sPLA2 Type V ELISA Kit
缩写：PLA2G5
Uniprot No.：P39877
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.18 ng/mL-12 ng/mL
灵敏度：0.04 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 84-102 | |||||
| 1:20 | Average % | 104 | ||||
| Range % | 100-110 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human PLA2G5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected122.2072.1502.1792.02361.7681.8011.7851.62931.2021.1841.1931.0371.50.6830.6340.6590.5030.750.4030.4280.4160.2600.3750.3210.3010.3110.1550.180.2450.2270.2360.08000.1590.1520.156 | ||||||
货期：3-5 working days
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