| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3K27ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J |
| Entrez GeneID | 8350 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Acetyl-Histone H3 (Lys27)抗体的3篇参考文献，按文献名称、作者和摘要内容分类整理：
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1. **文献名称**: "Histone H3K27ac separates active from poised enhancers and predicts developmental state"
**作者**: Creyghton, M.P. 等
**摘要**: 该研究利用H3K27ac抗体通过ChIP-seq技术，区分了活性增强子与静息态增强子，发现H3K27ac标记与细胞发育阶段密切相关，为表观遗传调控提供了关键分子标志。
2. **文献名称**: "Global mapping of H3K27ac in cancer reveals oncogenic reprogramming of enhancer landscapes"
**作者**: Zhang, X. 等
**摘要**: 研究通过H3K27ac抗体分析多种癌症模型，揭示了肿瘤细胞中增强子区域乙酰化异常，表明H3K27ac在癌基因激活和肿瘤发生中起重要作用。
3. **文献名称**: "Dynamic acetylation of histone H3K27 marks developmental transitions in mammalian chromatin"
**作者**: Tie, F. 等
**摘要**: 该文献使用H3K27ac抗体探究胚胎干细胞分化过程中组蛋白修饰的动态变化，发现其与关键发育基因的时序性激活直接相关，为细胞命运决定提供机制解释。
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这些研究均通过H3K27ac抗体揭示该修饰在基因调控、疾病机制及发育生物学中的功能，展示了该抗体在染色质分析中的广泛应用。
The Acetyl-Histone H3 (Lys27) antibody is a key tool for studying epigenetic regulation, specifically targeting the acetylation of lysine 27 on histone H3 (H3K27ac). Histone acetylation is a post-translational modification critical for chromatin structure and gene expression regulation. By neutralizing the positive charge of lysine residues, acetylation reduces histone-DNA interactions, promoting an open chromatin state that facilitates transcriptional activation. H3K27ac is enriched at active enhancers and promoters, serving as a hallmark of regulatory elements involved in cell-specific gene programs. Unlike H3K27 methylation (e.g., H3K27me3. associated with Polycomb-mediated gene silencing), H3K27ac is linked to transcriptional activation and is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs).
This antibody is widely used in chromatin immunoprecipitation (ChIP) assays to map genomic regions associated with active transcription, as well as in techniques like Western blotting and immunofluorescence to assess acetylation levels in various biological contexts. Dysregulation of H3K27ac has been implicated in diseases such as cancer, where aberrant enhancer activity drives oncogene expression, and in neurological disorders. Researchers rely on validated Acetyl-Histone H3 (Lys27) antibodies to explore epigenetic mechanisms, evaluate drug effects targeting acetylation pathways, and identify biomarkers for disease diagnosis or therapy. Its specificity and reliability make it essential for studies linking chromatin dynamics to cellular function and disease.
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