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产品描述：人磷脂酶A2(PLA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLA2G1B含量。PLA2G1B是一种磷脂酶A2。它在脂质代谢、炎症反应等过程中起关键作用。研究聚焦其水解磷脂产生脂肪酸和溶血磷脂机制，探索其在代谢性疾病、炎症相关疾病中的作用，有望为相关疾病药物研发提供新靶点与思路。试剂盒检测范围为0.45 ng/mL-30 ng/mL，能够支持基础医学研究中PLA2相关信号通路机制探索，适用于心血管疾病模型、神经退行性疾病模型或代谢综合征等领域的蛋白表达水平检测，也可用于体外药物筛选实验中PLA2活性调控化合物的作用效果评估，为相关生物标志物研究及新型治疗策略开发提供实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Group IB phospholipase A2 ELISA Kit; Group IB secretory phospholipase A2 ELISA Kit; MGC119834 ELISA Kit; MGC119835 ELISA Kit; PA21B_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit; Phospholipase A2 ELISA Kit; Phospholipase A2 group IB (pancreas) ELISA Kit; Phospholipase A2 group IB ELISA Kit; PLA 2 ELISA Kit; PLA2 ELISA Kit; PLA2A ELISA Kit; PLA2G1B ELISA Kit; PPLA 2 ELISA Kit; PPLA2 ELISA Kit; sPLA2IB ELISA Kit
缩写：PLA2G1B
Uniprot No.：P04054
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:10 | Average % | 104 | ||||
| Range % | 100-109 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:40 | Average % | 85 | ||||
| Range % | 81-92 | |||||
回收率：
| The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 93-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.5342.4522.4932.384151.6521.5761.6141.5057.51.0661.0041.0350.9263.750.5780.5610.5700.4611.80.3870.4010.3940.2850.90.2380.2450.2420.1330.450.1790.1870.1830.07400.1050.1120.109 | ||||||
货期：3-5 working days
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