| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Preb; SEC12;;PREB |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PREB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PREB抗体的虚构参考文献示例，供参考：
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1. **文献名称**: "PREB Antibody Application in Transcriptional Regulation Studies of Prolactin Signaling"
**作者**: Tanaka, K. et al.
**摘要**: 本研究使用特异性PREB抗体，通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了PREB蛋白在催乳素信号通路中作为转录调控因子的作用机制。结果表明，PREB与特定DNA元件结合，调控下游基因表达，可能影响乳腺细胞分化。
2. **文献名称**: "Altered PREB Expression in Breast Cancer Tissues Detected by Immunohistochemistry"
**作者**: Martinez, R. & Lee, S.
**摘要**: 通过免疫组化(IHC)和PREB抗体分析乳腺癌组织样本，发现PREB蛋白表达水平与肿瘤分级呈负相关。研究提示PREB可能作为抑癌因子，其低表达与患者预后不良相关。
3. **文献名称**: "Interaction of PREB with RNA Polymerase II: A Co-Immunoprecipitation Study"
**作者**: Gupta, A. et al.
**摘要**: 利用PREB抗体进行免疫共沉淀(Co-IP)实验，证实PREB与RNA聚合酶II的亚基存在直接互作，表明其在转录延伸阶段可能发挥调控功能，为基因表达机制提供了新见解。
4. **文献名称**: "Developmental Stage-Specific Expression of PREB in Mouse Embryonic Tissues"
**作者**: Chen, X. & Wang, Y.
**摘要**: 采用PREB抗体的免疫荧光技术，系统分析了小鼠胚胎发育过程中PREB的时空表达模式。结果显示，PREB在神经管和肝脏前体细胞中高表达，提示其可能在器官形成中起关键作用。
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**注**：以上文献为示例性内容，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。如需进一步协助查找真实文献，请提供更多关键词或研究方向。
PREB (Prolactin Regulatory Element-Binding protein) is a transcription factor initially identified for its role in regulating prolactin gene expression by binding to a specific element in the prolactin promoter. It belongs to the WD40 repeat protein family, characterized by conserved β-propeller structures that mediate protein-protein interactions. PREB functions as a regulatory component in secretory pathways, influencing processes like vesicle trafficking and hormone secretion, particularly in pituitary cells. Beyond prolactin regulation, it has been implicated in broader cellular functions, including endoplasmic reticulum stress response, lipid metabolism, and adipocyte differentiation.
PREB antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes within PREB's N-terminal or C-terminal regions. They are widely used in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate PREB's role in transcriptional regulation and cellular homeostasis. Dysregulation of PREB has been linked to metabolic disorders, obesity, and certain cancers, making its antibodies valuable for both basic research and clinical investigations. Validation of PREB antibodies includes testing for specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection in diverse biological samples.
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