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产品描述：人XIIA组分泌型磷脂酶A2(PLA2G12A)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLA2G12A含量。PLA2G12A是一种分泌型磷脂酶A2，具有低活性，主要在心脏、骨骼肌、肾脏、肝脏和胰腺中表达。其研究机制涉及磷脂代谢和类二十烷酸的产生，与多种生理和病理作用相关，如心血管疾病和神经发育障碍。试剂盒检测范围为4.7 pg/mL-300 pg/mL，该产品可应用于细胞脂质代谢研究、炎症相关信号通路探索、疾病动物模型生物标志物分析等科研场景，为探究PLA2G12A在病理生理过程中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 3.1.1.4 ELISA Kit; Group XII secreted phospholipase A2 ELISA Kit; Group XIIA secreted phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 precursor ELISA Kit; GXII ELISA Kit; GXII sPLA2 ELISA Kit; PG12A_HUMAN ELISA Kit; phosphatidylcholine 2 acylhydrolase 12A ELISA Kit; Phosphatidylcholine 2 acylhydrolase GXII ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit; Phospholipase A2 group XIIA ELISA Kit; PLA2G12 ELISA Kit; Pla2g12a ELISA Kit; ROSSY ELISA Kit; sPLA2 XII ELISA Kit; sPLA2-XII ELISA Kit
缩写：PLA2G12A
Uniprot No.：Q9BZM1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.2 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 93 | |||||
| Range % | 90-96 | ||||||
| 1:40 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:80 | Average % | 86 | |||||
| Range % | 83-90 | ||||||
| 1:160 | Average % | 101 | |||||
| Range % | 98-106 | ||||||
回收率：
| The recovery of human PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 80-90 | |||||
| EDTA plasma (n=4) | 101 | 97-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average 3000.2360.2300.233 1500.3860.3910.389 750.5890.5920.591 37.50.8470.8340.841 18.751.0761.1001.088 9.41.5341.5021.518 4.71.9521.9911.972 02.3452.4012.373 | |||||||
货期：3-5 working days
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