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产品描述：人凝血酶原前体蛋白(PIVKA-II)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、腹水、组织匀浆样本中的PIVKA-II含量。PIVKA-II即异常凝血酶原，是一种肿瘤标志物。在维生素K缺乏或拮抗时产生，在肝癌等肿瘤细胞中高表达。研究机制上，它可反映肝细胞癌的生长和凝血功能异常，为肝癌早期诊断、病情监测和预后评估提供重要参考。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于肝癌发生机制研究、抗肿瘤药物研发、肝脏功能评估等科研领域，其多样本兼容性特别适合临床前研究中不同生物样本的横向对比分析，如肿瘤组织匀浆中异常凝血酶原表达检测，或腹水样本中的肿瘤标志物水平动态监测，为维生素K代谢研究及肝病相关分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PIVKA-II
种属：Homo sapiens (Human)
样本类型：serum, plasma, ascitic fluid, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 86-101 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-108 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-103 | |||||
回收率：
| The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-104 | ||||
| EDTA plasma (n=4) | 97 | 91-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.4672.4182.4432.327 101.9621.9451.9541.838 51.3421.3111.3271.211 2.50.8230.7720.7980.682 1.250.5040.4960.5000.384 0.6250.3310.3210.3260.210 0.3120.2010.1890.1950.079 00.1170.1140.116 | ||||||
货期：3-5 working days
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