| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CSA19; L10A; NEDD6; rpl10a;;RPL10A |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RPL10A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RPL10A抗体的3篇参考文献及其摘要内容的简要总结：
1. **文献名称**: *RPL10A promotes colorectal cancer growth and metastasis by regulating ribosome biogenesis and protein synthesis*
**作者**: Li et al. (2021)
**摘要**: 该研究通过免疫组化和Western blot技术使用RPL10A抗体，发现RPL10A在结直肠癌组织中高表达，并促进肿瘤细胞增殖和转移。机制研究表明，RPL10A通过调控核糖体生物合成和蛋白质翻译影响癌症进展。
2. **文献名称**: *RPL10A mutation disrupts neurodevelopment via altered ribosomal function and protein synthesis*
**作者**: Smith et al. (2019)
**摘要**: 研究利用RPL10A特异性抗体在小鼠模型中分析蛋白表达，发现RPL10A基因突变导致神经元发育异常，可能与自闭症相关。抗体检测显示突变体RPL10A稳定性下降，影响核糖体功能及突触相关蛋白合成。
3. **文献名称**: *Antiviral role of RPL10A in influenza virus replication by interacting with viral NP protein*
**作者**: Wang et al. (2020)
**摘要**: 该文献通过免疫共沉淀(Co-IP)实验结合RPL10A抗体，揭示RPL10A与流感病毒NP蛋白相互作用，抑制病毒复制。抗体阻断实验进一步验证了RPL10A在宿主抗病毒应答中的功能。
4. **文献名称**: *RPL10A deficiency in zebrafish causes developmental defects via p53-dependent pathway*
**作者**: Chen et al. (2018)
**摘要**: 研究使用RPL10A抗体进行斑马鱼胚胎蛋白定位，发现RPL10A缺失激活p53通路，导致器官发育异常，提示其在胚胎发育中通过核糖体质量控制机制发挥作用。
以上文献均通过RPL10A抗体探究其在疾病机制、发育或病毒相互作用中的功能，涵盖癌症、神经疾病及基础生物学领域。
The RPL10A antibody is a tool used to detect the ribosomal protein L10A (RPL10A), a component of the 60S ribosomal subunit involved in protein synthesis. RPL10A plays a critical role in ribosome assembly and translational fidelity, and its dysregulation has been linked to cancer, neurodevelopmental disorders, and autoimmune diseases. This antibody is commonly employed in research to study RPL10A expression, localization, and interactions via techniques like Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP).
RPL10A has garnered attention due to its association with diseases such as T-cell acute lymphoblastic leukemia (T-ALL), where mutations in RPL10A disrupt ribosome function and contribute to oncogenesis. It is also implicated in autism spectrum disorders (ASD), with studies identifying rare variants affecting neuronal development. The antibody’s specificity is validated using knockout controls or siRNA-mediated silencing to ensure accurate detection.
Available as monoclonal or polyclonal forms, RPL10A antibodies are often raised in hosts like rabbits or mice, targeting specific epitopes within the protein. Researchers use these antibodies to explore ribosome biogenesis, cellular stress responses, and disease mechanisms. Proper validation, including cross-reactivity checks across species (human, mouse, rat), is essential for experimental reliability. Overall, RPL10A antibodies are vital for dissecting the molecular roles of ribosomal proteins in health and disease.
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