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产品描述：人蛋白(肽酰脯氨酰顺/反异构酶)NIMA结合1(PIN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的PIN1含量。PIN1 是一种肽基脯氨酰顺反异构酶，在细胞的增殖和存活中起重要作用。其异常表达与肿瘤、神经退行性疾病等密切相关。研究发现它能通过影响底物蛋白构象和功能参与多条信号通路，成为潜在的疾病治疗靶点。试剂盒检测范围为1.56 pg/mL-100 pg/mL，本试剂盒检测灵敏度为1.56 pg/mL，线性范围为1.56 - 100 pg/mL，可精准检测微量样本中的PIN1含量，适用于癌症生物学、神经科学等基础研究领域，为探索PIN1在疾病微环境中的作用、药物靶点筛选及分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DOD ELISA Kit; DODO, Drosophila, homolog of ELISA Kit; FLJ40239 ELISA Kit; FLJ77628 ELISA Kit; MGC10717 ELISA Kit; NIMA interacting 1 ELISA Kit; Peptidyl prolyl cis trans isomerase NIMA interacting 1 ELISA Kit; Peptidyl prolyl cis/trans isomerase NIMA interacting ELISA Kit; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 ELISA Kit; Peptidyl-prolyl cis-trans isomerase pin1 ELISA Kit; Peptidylprolyl cis/trans isomerase NIMA interacting 1 ELISA Kit; Pin 1 ELISA Kit; Pin1 ELISA Kit; PIN1_HUMAN ELISA Kit; PPIase Pin1 ELISA Kit; Prolyl isomerase ELISA Kit; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 ELISA Kit; Protein NIMA interacting 1 ELISA Kit; Rotamase Pin1 ELISA Kit; UBL 5 ELISA Kit; UBL5 ELISA Kit
缩写：PIN1
Uniprot No.：Q13526
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PIN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:80 | Average % | 102 | ||||
| Range % | 98-108 | |||||
| 1:160 | Average % | 89 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of human PIN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 101 | 96-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5632.4622.5132.388502.0032.1042.0541.929251.5001.5111.5061.38112.51.0431.0541.0490.9246.250.6240.6130.6190.4943.120.3710.3600.3660.2411.560.2440.2380.2410.11600.1250.1240.125 | ||||||
货期：3-5 working days
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