| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gprc5b; RAIG2;;GPRC5B |
| WB Predicted band size | Calculated MW: 45 kDa ; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human GPRC5B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GPRC5B抗体的3篇示例参考文献(注：以下为虚拟构造的示例，实际文献需通过学术数据库检索获取)：
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1. **文献名称**: "GPRC5B modulates hepatic lipid metabolism through antibody-validated protein interactions"
**作者**: Li, X. et al.
**摘要**: 本研究利用特异性GPRC5B抗体，通过免疫共沉淀和Western blot技术，揭示了GPRC5B在肝脏脂质代谢中的调控作用，发现其与PPARγ通路存在直接互作，为代谢性疾病治疗提供新靶点。
2. **文献名称**: "Prognostic value of GPRC5B overexpression in colorectal cancer detected by immunohistochemistry"
**作者**: Wang, Y. et al.
**摘要**: 通过免疫组化技术结合GPRC5B特异性抗体，分析了结直肠癌组织中GPRC5B的表达水平，证实其高表达与患者生存率降低及肿瘤转移显著相关。
3. **文献名称**: "Development and validation of a novel monoclonal antibody for GPRC5B in pancreatic β-cell function studies"
**作者**: Tanaka, K. et al.
**摘要**: 报道了一种高特异性抗GPRC5B单克隆抗体的开发，并利用该抗体证实了GPRC5B在胰岛β细胞胰岛素分泌中的关键作用，为糖尿病机制研究提供工具支持。
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如需真实文献，建议在PubMed、Google Scholar等平台检索关键词“GPRC5B antibody”、“GPRC5B immunohistochemistry”或结合具体研究领域(如癌症、代谢疾病)进行筛选。
GPRC5B (G Protein-Coupled Receptor Class C Group 5 Member B) is an orphan receptor within the GPCR superfamily, characterized by a seven-transmembrane domain structure and extracellular ligand-binding regions. Though its endogenous ligand remains unidentified, GPRC5B is implicated in lipid metabolism, adipose tissue regulation, and cellular differentiation. Studies link it to metabolic disorders, neurodegenerative diseases, and cancers, particularly through interactions with signaling pathways like Wnt/β-catenin and EGFR.
GPRC5B antibodies are critical tools for detecting and quantifying the receptor in techniques such as Western blotting, immunohistochemistry, and flow cytometry. These antibodies typically target extracellular or intracellular epitopes, enabling research into its tissue-specific expression, subcellular localization, and disease-related dysregulation. Commercial antibodies are often validated for cross-reactivity and specificity across species, though challenges persist due to structural similarities with other GPCR family members.
Current research leverages GPRC5B antibodies to explore its role in obesity, insulin resistance, and tumor progression. Recent findings suggest it may modulate lipid storage in adipocytes and influence cancer cell proliferation. However, functional ambiguity persists, driving demand for high-quality antibodies to dissect its mechanistic pathways and therapeutic potential.
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