| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NPAP60; NPAP60L; Nup50;;NUP50 |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NUP50 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NUP50抗体的3-4篇参考文献的示例(内容为虚构，供参考)：
1. **文献名称**: "NUP50 regulates nuclear import through direct interaction with importin-α"
**作者**: Mackay, D.R., et al.
**摘要**: 本研究利用特异性NUP50抗体，通过免疫共沉淀和免疫荧光技术，揭示了NUP50通过与importin-α结合调控核质运输的分子机制，并发现其缺失导致细胞周期阻滞。
2. **文献名称**: "The role of NUP50 in mitotic progression and chromosomal stability"
**作者**: Rodriguez-Bravo, V., et al.
**摘要**: 通过Western blot和免疫染色技术(使用NUP50抗体)，作者证明NUP50在维持有丝分裂纺锤体完整性中起关键作用，其敲低引发染色体错配和细胞凋亡。
3. **文献名称**: "NUP50 interacts with nuclear pore components to maintain permeability barrier function"
**作者**: Lussi, Y.C., et al.
**摘要**: 利用NUP50抗体进行超分辨率显微成像，研究发现NUP50与NUP153等核孔蛋白形成复合物，调控核膜选择性通透性，并影响大分子核质交换效率。
4. **文献名称**: "NUP50 expression correlates with poor prognosis in breast cancer"
**作者**: Xu, H., et al.
**摘要**: 基于NUP50抗体的免疫组化分析显示，NUP50在乳腺癌组织中高表达，且与肿瘤转移和患者生存率降低显著相关，提示其作为潜在生物标志物的价值。
(注：以上文献为示例，实际引用需以真实发表的论文为准。)
NUP50 (Nucleoporin 50) is a component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport. As a member of the nucleoporin family, NUP50 plays a critical role in mediating the selective transport of macromolecules between the nucleus and cytoplasm. Structurally, it contains distinct N-terminal and C-terminal domains, with the latter interacting directly with nuclear transport receptors like Importin-α and CRM1 to facilitate cargo release or binding during transport cycles. Beyond its role in trafficking, NUP50 is implicated in mitotic progression, chromatin organization, and gene expression regulation.
Antibodies targeting NUP50 are essential tools for studying its localization, expression dynamics, and functional interactions. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate NPC architecture, nuclear transport mechanisms, and cell cycle-dependent changes. Dysregulation of NUP50 has been linked to diseases, including certain cancers and cardiovascular disorders, making its detection and analysis clinically relevant. For instance, altered NUP50 expression levels are observed in breast cancer and leukemia, suggesting potential diagnostic or therapeutic applications. Researchers rely on well-validated NUP50 antibodies to explore its molecular interactions, post-translational modifications, and tissue-specific distribution, contributing to a deeper understanding of NPC biology and disease pathogenesis.
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