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Human Profilin 1 ELISA Kit

  • 中文名： 人前纤维蛋白1ELISA Kit
货号: IPD-E16491h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人前纤维蛋白1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PFN1含量。PFN1 即 Profilin 1，是一种在细胞内广泛分布的肌动蛋白结合蛋白。它可调节肌动蛋白聚合，在细胞迁移、增殖、存活等方面发挥关键作用。当前针对 PFN1 在肿瘤发生发展、神经退行性疾病等机制中的研究较多，以期为相关疾病治疗提供新靶点。试剂盒检测范围为0.78 ng/ml- 50 ng/ml，适用于细胞生物学、病理机制等科研领域的研究；可为探究Profilin 1在疾病模型中的功能、信号通路交互作用以及生物标志物筛选提供可靠工具，特别适用于肿瘤生物学、炎症反应或神经退行性疾病等相关课题的体外实验研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Actin binding protein ELISA Kit; ALS18 ELISA Kit; Epididymis tissue protein Li 184a ELISA Kit; OTTHUMP00000125244 ELISA Kit; PFN 1 ELISA Kit; Pfn ELISA Kit; PFN1 ELISA Kit; PROF1_HUMAN ELISA Kit; Profilin I ELISA Kit; Profilin-1 ELISA Kit; Profilin1 ELISA Kit; ProfilinI ELISA Kit
  • 缩写：
    PFN1
  • Uniprot No.：
    P07737
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/ml - 50 ng/ml
  • 灵敏度：
    0.195 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human profilin 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    106


    Range %

    100-111


    1:2

    Average %

    96


    Range %

    89-103


    1:4

    Average %

    99


    Range %

    92-105


    1:8

    Average %

    102


    Range %

    97-105


  • 回收率：

    The recovery of human profilin 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    91-100


    EDTA plasma (n=4)

    90

    86-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    50

    2.539

    2.634

    2.587

    2.455


    25

    1.835

    1.767

    1.801

    1.670


    12.5

    1.199

    1.158

    1.178

    1.047


    6.25

    0.732

    0.729

    0.731

    0.599


    3.12

    0.452

    0.431

    0.442

    0.310


    1.56

    0.297

    0.303

    0.300

    0.168


    0.78

    0.215

    0.209

    0.212

    0.081


    0

    0.134

    0.129

    0.132




  • 货期：
    3-5 working days

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