首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板因子4(PF-4/CXCL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PF4含量。PF4即血小板因子4，是血小板α颗粒分泌的一种小分子蛋白。在生理上它能趋化炎症细胞、参与凝血等。研究机制方面，其在血栓形成、肿瘤微环境调节等过程有作用，深入研究它对相关疾病治疗靶点的开发具有重要意义。试剂盒检测范围为0.156 μg/mL-10 μg/mL，灵敏度为0.039 μg/mL。科研人员可将其应用于血小板活化机制、血栓相关疾病模型或炎症微环境等研究领域，为探索PF - 4/CXCL4在病理生理过程中的调控作用提供可靠工具。试剂盒兼容多种生物样本类型，满足实验室基础研究及药物开发中的靶点验证需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
缩写：PF4
Uniprot No.：P06765
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.039 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-104 | |||||
回收率：
| The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 83-99 | ||||
| EDTA plasma (n=4) | 97 | 89-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected102.6842.6172.6512.46352.2042.1862.1952.0072.51.5631.6081.5861.3981.250.8810.8990.8900.7020.6250.4800.4910.4860.2980.3120.3450.3370.3410.1530.1560.2470.2500.2490.06100.1850.1900.188 | ||||||
货期：3-5 working days
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