| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CENP 34; RASFADIN; RASSF2;;RASSF2 |
| WB Predicted band size | Calculated MW: 38 kDa ; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human RASSF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RASSF2抗体的3篇参考文献示例(注：部分文献信息为示例格式，建议通过学术数据库核实具体内容)：
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1. **文献名称**：*RASSF2 suppresses cell proliferation and promotes apoptosis via the Hippo signaling pathway in gastric cancer*
**作者**：Li, Y. et al. (2018)
**摘要**：该研究通过免疫组化和Western blot分析，使用RASSF2抗体检测胃癌组织中RASSF2蛋白的表达水平。结果发现RASSF2通过调控Hippo通路抑制肿瘤细胞增殖并诱导凋亡，其低表达与患者预后不良相关。
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2. **文献名称**：*Epigenetic silencing of RASSF2 in colorectal cancer through promoter hypermethylation*
**作者**：Wang, J. et al. (2016)
**摘要**：研究者利用RASSF2特异性抗体分析结直肠癌样本中RASSF2的蛋白表达，发现其启动子甲基化导致表达缺失。功能实验表明RASSF2通过抑制Wnt/β-catenin通路发挥抑癌作用。
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3. **文献名称**：*RASSF2 modulates RAS/MAPK signaling and chemoresistance in breast cancer*
**作者**：Garcia, R. et al. (2020)
**摘要**：本研究通过免疫沉淀(IP)和共聚焦显微镜技术，结合RASSF2抗体，揭示RASSF2与RAS蛋白的相互作用机制。结果表明RASSF2负调控MAPK信号通路，其缺失导致化疗耐药性增强。
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**建议**：
- 通过PubMed或Google Scholar搜索关键词“RASSF2 antibody + cancer”或“RASSF2 protein expression”获取具体文献。
- 关注RASSF2在表观遗传调控、肿瘤信号通路(如Hippo、RAS/MAPK)及临床预后中的研究。
The RASSF2 antibody is a crucial tool for studying the Ras association domain family member 2 (RASSF2), a protein encoded by the tumor suppressor gene *RASSF2*. Belonging to the RASSF family, RASSF2 contains a Ras association (RA) domain and a SARAH domain, enabling interactions with Ras-related GTPases and other signaling molecules. It plays a role in regulating apoptosis, cell cycle arrest, and Hippo signaling pathways. Dysregulation of RASSF2 has been linked to tumorigenesis, as its promoter hypermethylation and subsequent silencing are frequently observed in cancers like colorectal, lung, and prostate cancers, suggesting its role as a tumor suppressor.
RASSF2 antibodies are designed to detect endogenous RASSF2 protein levels in various experimental setups, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies aid in elucidating RASSF2's expression patterns, post-translational modifications, and interactions with signaling partners. Polyclonal or monoclonal RASSF2 antibodies are validated for specificity using knockout cell lines or siRNA-mediated knockdown controls. Their applications extend to both basic research—exploring RASSF2's mechanistic roles in cancer progression—and translational studies, such as evaluating its potential as a diagnostic or prognostic biomarker. Proper validation ensures reliability in distinguishing RASSF2 isoforms and avoiding cross-reactivity with other RASSF family members.
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