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产品描述：人蛋白二硫化物异构酶A3(PDIA3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的PDIA3含量。试剂盒检测范围为1.25 ng/mL-80 ng/mL，该产品适用于基础科研领域，包括探究PDIA3在代谢性疾病、免疫调节及肿瘤微环境中的功能机制，或用于细胞实验中监测内质网应激状态下PDIA3的动态分泌水平。实验操作流程标准化，提供完整配套试剂，满足体外生物学研究中蛋白质定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：58 kDa glucose regulated protein ELISA Kit; 58 kDa glucose-regulated protein ELISA Kit; 58 kDa microsomal protein ELISA Kit; Disulfide isomerase ER 60 ELISA Kit; Disulfide isomerase ER-60 ELISA Kit; Endoplasmic reticulum resident protein 57 ELISA Kit; Endoplasmic reticulum resident protein 60 ELISA Kit; ER p57 ELISA Kit; ER protein 57 ELISA Kit; ER protein 60 ELISA Kit; ERp 57 ELISA Kit; ERp57 ELISA Kit; ERp60 ELISA Kit; ERp61 ELISA Kit; Glucose Regulated Protein 58 Kd ELISA Kit; GRP 57 ELISA Kit; GRP 58 ELISA Kit; GRP57 ELISA Kit; HsT17083 ELISA Kit; p58 ELISA Kit; PDIA 3 ELISA Kit; PDIA3 ELISA Kit; PDIA3_HUMAN ELISA Kit; Phospholipase C alpha ELISA Kit; PI PLC ELISA Kit; Protein disulfide isomerase A3 ELISA Kit; Protein disulfide isomerase family A member 3 ELISA Kit; Protein disulfide-isomerase A3 ELISA Kit
缩写：PDIA3
Uniprot No.：P30101
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 98-108 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of hhuman PDIA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.5762.5212.5492.398402.1562.0542.1051.954201.4871.4451.4661.315100.7660.7770.7720.62150.4920.4680.4800.3292.50.3740.3690.3720.2211.250.2550.2480.2520.10100.1530.1490.151 | ||||||
货期：3-5 working days
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