首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板衍生生长因子DD(PDGF-DD) 酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-DD含量。PDGF - DD是一种血小板衍生生长因子异构体。它在细胞的生长、增殖和迁移等过程中发挥重要作用。其研究机制主要聚焦于与受体结合后激活下游信号通路，影响细胞行为，在肿瘤、纤维化等疾病的发生发展中可能有潜在影响，是药物研发的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域探究PDGF-DD在大鼠心血管疾病模型、器官纤维化机制、创伤修复实验中的动态表达特征，通过血清或血浆样本可系统性评估循环系统中PDGF-DD水平，而组织匀浆检测则有助于解析特定器官或病变部位的局部蛋白表达模式，为相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-DD
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-103 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7442.6782.7112.53152.1392.1122.1261.9462.51.5091.4761.4931.3131.251.0251.0031.0140.8340.6250.6620.6510.6570.4770.3120.4030.3860.3950.2150.1560.2920.2720.2820.10200.1810.1790.180 | ||||||
货期：3-5 working days
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