首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血小板衍生生长因子受体β(PDGFRB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGFRB含量。PDGFRB即血小板衍生生长因子受体β，在细胞的生长、增殖和迁移等过程中有重要作用。异常激活或表达与肿瘤血管生成、肿瘤细胞增殖及间质纤维化等多种病理过程相关。目前研究聚焦其信号通路机制，以开发针对性的靶向治疗药物。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。本产品适用于基础医学研究中PDGFRB相关信号通路的机制探索、疾病模型中的蛋白表达水平评估，以及药物干预实验的生物标志物检测，为细胞生物学、病理机制研究和靶向治疗开发提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta platelet derived growth factor receptor ELISA Kit; Beta-type platelet-derived growth factor receptor ELISA Kit; CD 140B ELISA Kit; CD140 antigen-like family member B ELISA Kit; CD140b ELISA Kit; CD140b antigen ELISA Kit; IBGC4 ELISA Kit; IMF1 ELISA Kit; JTK12 ELISA Kit; OTTHUMP00000160528 ELISA Kit; PDGF R beta ELISA Kit; PDGF Receptor beta ELISA Kit; PDGF-R-beta ELISA Kit; PDGFR 1 ELISA Kit; PDGFR ELISA Kit; PDGFR beta ELISA Kit; PDGFR1 ELISA Kit; PDGFRB ELISA Kit; PGFRB_HUMAN ELISA Kit; Platelet derived growth factor receptor 1 ELISA Kit; Platelet derived growth factor receptor beta ELISA Kit; Platelet derived growth factor receptor beta polypeptide ELISA Kit
缩写：PDGFRB
Uniprot No.：P09619
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDGFRB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human PDGFRB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-97 | ||||
| EDTA plasma (n=4) | 108 | 102-113 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.5752.5212.5482.37120002.1282.1842.1561.97910001.6751.5861.6311.4545001.0671.0471.0570.8802500.6840.6780.6810.5041250.4680.4980.4830.30662.50.2920.2870.2900.11300.1790.1750.177 | ||||||
货期：3-5 working days
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