首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血小板衍生生长因子CC(PDGF-CC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的PDGF-CC含量。PDGF-CC 即血小板衍生生长因子 CC，在细胞生长、增殖等生理过程中发挥作用。其异常表达与肿瘤、纤维化等疾病的发生发展相关。研究机制主要聚焦于其信号通路，阻断该信号有望为相关疾病治疗提供方向，目前是研究热门靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，科研人员可通过该产品开展PDGF-CC在疾病模型中的表达水平监测、药物干预效果评估或信号通路机制研究，为心血管生物学、肿瘤微环境及再生医学等领域的体外实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-CC
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-95 | |||||
回收率：
| The recovery of human PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.3082.4182.3632.26051.8872.0321.9601.8572.51.4521.4231.4381.3351.250.7820.8210.8020.6990.6250.4770.4970.4870.3840.3120.3250.3460.3360.2330.1560.2150.2050.2100.10700.1040.1010.103 | ||||||
货期：3-5 working days
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