首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板衍生生长因子CC(PDGF-CC) 酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-CC含量。PDGF - CC即血小板衍生生长因子CC，是PDGF家族成员。它在胚胎发育、组织修复等过程有重要作用。其异常表达与肿瘤、纤维化等疾病相关。研究机制多围绕其与受体结合，激活下游信号通路，影响细胞增殖、迁移等方面。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域对大鼠模型中PDGF-CC蛋白表达的定量分析，例如在心血管疾病、组织再生或肿瘤机制研究中评估目标分子动态变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-CC
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 99-112 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of rat PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 82-93 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.1432.0032.0731.8935001.4871.4051.4461.2662500.8990.9270.9130.7331250.6030.5690.5860.40662.50.3910.4120.4020.22231.20.2700.2840.2770.09715.60.2400.2310.2360.05600.1830.1760.180 | |||||||
货期：3-5 working days
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