首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管内皮细胞生长因子E(VEGF-E)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的PDGFC含量。PDGFC即血小板衍生生长因子C，是在组织发育、修复等过程中发挥重要作用的分泌型生长因子。其研究机制主要围绕其与受体结合后激活细胞内信号传导通路，调控细胞增殖、迁移等，深入研究有助于为相关疾病的治疗提供新靶点与策略。试剂盒检测范围为6.25 pg/mL-400 pg/mL，支持科研人员在体外实验中定量分析VEGF - E的表达动态，可广泛应用于细胞模型构建、疾病机制探索、药物干预效果评估等基础研究，其宽泛的线性范围和多样本兼容性为血管生物学、肿瘤学等领域的体外实验提供了可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fallotein ELISA Kit; hSCDGF ELISA Kit; PDGF-C ELISA Kit; PDGFC ELISA Kit; PDGFC latent form ELISA Kit; PDGFC receptor-binding form ELISA Kit; PDGFC_HUMAN ELISA Kit; Platelet derived growth factor C ELISA Kit; Platelet-derived growth factor C; receptor-binding form ELISA Kit; SCDGF ELISA Kit; Secretory growth factor like protein ELISA Kit; Secretory growth factor like protein fallotein ELISA Kit; Spinal cord derived growth factor ELISA Kit; Spinal cord-derived growth factor ELISA Kit; VEGF E ELISA Kit; VEGF-E ELISA Kit
缩写：PDGFC
Uniprot No.：Q9NRA1
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of human VEGF-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 83-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.8062.7122.7592.6592002.0122.0662.0391.9391001.4731.3841.4291.329500.9580.8930.9260.826250.4940.4730.4840.38412.50.3210.3010.3110.2116.250.2540.2490.2520.15200.1020.0970.100 | ||||||
货期：3-5 working days
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