首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板衍生生长因子BB(PDGF-BB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PDGF-BB含量。PDGF-BB属于血小板衍生生长因子，在细胞的生长、增殖和分化中发挥重要作用。其异常表达与肿瘤血管生成、纤维化疾病等多种病理过程相关。研究通过抑制其信号通路，阻断细胞增殖与迁移，有望为相关疾病治疗提供新策略。试剂盒检测范围为39.063 pg/mL-2500 pg/mL，该产品适用于基础科研领域，如细胞模型中生长因子调控机制研究、动物疾病模型中PDGF - BB动态变化的定量分析，以及组织微环境修复相关实验的分子水平检测，为探索心血管疾病、组织再生及病理损伤等研究方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-BB
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：39.063 pg/mL-2500 pg/mL
灵敏度：9.77 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 104 | ||||
| Range % | 101-107 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 94-100 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 83-90 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 101-109 | ||||
| EDTA plasma (n=4) | 110 | 107-113 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected25002.7942.7532.7742.57812502.2042.2002.2022.0066251.5301.5011.5161.320312.50.9010.9250.9130.717156.250.6350.6520.6440.44878.1250.4590.4350.4470.25139.0630.3760.3680.3720.17600.1970.1940.196 | ||||||
货期：3-5 working days
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