首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血小板衍生生长因子AA(PDGF-AA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AA含量。PDGF - AA 即血小板衍生生长因子 AA 型，是一种重要的细胞因子。它在细胞增殖、分化等过程中发挥关键作用，与组织修复、血管生成等生理过程相关。研究机制主要围绕其与受体结合，激活下游信号通路，在肿瘤、纤维化等疾病发病机制中有重要研究价值。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于心血管疾病机制研究、组织再生模型分析、抗纤维化药物开发等科研领域；为探索 PDGF - AA 在炎症反应、胚胎发育或创伤愈合中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PDGF-AA
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 90-102 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 92-104 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 85-99 | ||||||
回收率：
| The recovery of human PDGF-AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 84-94 | |||||
| EDTA plasma (n=4) | 104 | 95-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.3292.3682.3492.192101.6951.5761.6361.47950.9880.9530.9710.8142.50.6510.6470.6490.4921.250.3650.3550.3600.2030.6250.2570.2480.2530.0960.3120.2050.2110.2080.05100.1590.1550.157 | |||||||
货期：3-5 working days
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