首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人程序性死亡-1(PD-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDCD1含量。PDCD1 又称程序性死亡受体 1，是一种重要的免疫检查点蛋白。其背景在于可负调控免疫反应，避免免疫过度激活。研究机制方面，PDCD1 与其配体结合后，能抑制 T 细胞活化，肿瘤细胞常利用该机制逃避免疫监视，针对它的免疫疗法是抗癌研究热点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品广泛应用于免疫治疗机制研究、肿瘤微环境分析、免疫抑制模型构建等领域，为探索PD - 1在免疫调节网络中的功能、评估药物干预效果或生物标志物筛选提供可靠工具，特别适用于体外实验中PD - 1表达水平的动态监测及跨样本类型的对比研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD279 ELISA Kit; CD279 antigen ELISA Kit; hPD 1 ELISA Kit; hPD l ELISA Kit; hPD-1 ELISA Kit; hSLE1 ELISA Kit; PD 1 ELISA Kit; PD-1 ELISA Kit; PD1 ELISA Kit; PDCD 1 ELISA Kit; PDCD1 ELISA Kit; PDCD1_HUMAN ELISA Kit; Programmed cell death 1 ELISA Kit; Programmed cell death 1 protein ELISA Kit; Programmed cell death protein 1 ELISA Kit; Protein PD 1 ELISA Kit; Protein PD-1 ELISA Kit; SLEB2 ELISA Kit; Systemic lupus erythematosus susceptibility 2 ELISA Kit
缩写：PDCD1
Uniprot No.：Q15116
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PD-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of human PD-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 96-104 | ||||
| EDTA plasma (n=4) | 91 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6732.6172.6452.437 5002.3022.2392.2712.063 2501.8531.7141.7841.576 1251.5241.4291.4771.269 62.51.0570.9961.0270.819 31.20.6390.6820.6610.453 15.60.3070.3290.3180.110 00.2050.2100.208 | ||||||
货期：3-5 working days
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