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产品描述：人细胞程序性死亡6互动蛋白(PDCD6IP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDCD6IP含量。PDCD6IP即凋亡相关基因6相互作用蛋白。它参与细胞凋亡等多种生理过程。在研究机制上，其与多种信号通路相关，能影响细胞内囊泡运输、膜动态变化等，在肿瘤等疾病发生发展中可能扮演重要角色，是潜在研究靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，本产品适用于基础科研领域，如探究细胞凋亡信号通路调控机制、肿瘤微环境中囊泡介导的细胞间通讯研究，或用于评估体外培养细胞模型中PDCD6IP的表达水平，为相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIP1 ELISA Kit; ALG 2 interacting protein 1 ELISA Kit; ALG-2-interacting protein 1 ELISA Kit; ALG2 interacting protein X ELISA Kit; Alix ELISA Kit; Apoptosis linked gene 2 interacting protein X ELISA Kit; Dopamine receptor interacting protein 4 ELISA Kit; DRIP4 ELISA Kit; Hp95 ELISA Kit; KIAA1375 ELISA Kit; MGC17003 ELISA Kit; PDC6I_HUMAN ELISA Kit; PDCD6 interacting protein ELISA Kit; PDCD6-interacting protein ELISA Kit; PDCD6IP ELISA Kit; Programmed cell death 6 interacting protein ELISA Kit; Programmed cell death 6-interacting protein ELISA Kit
缩写：PDCD6IP
Uniprot No.：Q8WUM4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDCD6IP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 83-99 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 84-94 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 93-103 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 95-107 | ||||||
回收率：
| The recovery of human PDCD6IP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 96-107 | |||||
| EDTA plasma (n=4) | 89 | 84-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.5222.5542.5382.37015001.9842.0692.0271.8597501.4721.4641.4681.3003750.8490.8280.8390.671187.50.5140.4990.5070.339940.3560.3810.3690.201470.2390.2520.2460.07800.1680.1670.168 | |||||||
货期：3-5 working days
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