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Human Programmed cell death 1 ligand 2(PDCD1LG2) ELISA kit

  • 中文名： 人程序性细胞死亡1配体2(PDCD1LG2)|酶联免疫试剂盒
货号: IPD-EL017667HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人程序性细胞死亡1配体2(PDCD1LG2)|酶联免疫试剂盒，为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的PDCD1LG2含量。PDCD1LG2 即程序性死亡配体 2，是免疫检查点分子。其作为配体与受体结合可调控 T 细胞活性。在肿瘤免疫中，它能抑制机体免疫反应，使肿瘤逃避监视。目前对其研究聚焦于阻断该分子来增强抗肿瘤免疫，为肿瘤免疫治疗提供新思路。试剂盒检测范围为0.45 ng/ml-30 ng/ml.，可兼容多种生物样本类型，为研究肿瘤免疫治疗、感染性疾病或自身免疫性疾病中的PD-L2调控机制提供可靠工具，广泛应用于免疫学、肿瘤学等基础研究领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    B7 dendritic cell molecule ELISA Kit; B7-DC ELISA Kit; B7DC ELISA Kit; bA574F11.2 ELISA Kit; Btdc ELISA Kit; Butyrophilin B7 DC ELISA Kit; Butyrophilin B7-DC ELISA Kit; Butyrophilin B7DC ELISA Kit; CD 273 ELISA Kit; CD273 ELISA Kit; CD273 antigen ELISA Kit; MGC142238 ELISA Kit; MGC142240 ELISA Kit; PD 1 ligand 2 ELISA Kit; PD L2 ELISA Kit; PD-1 ligand 2 ELISA Kit; PD-L2 ELISA Kit; PD1 ligand 2 ELISA Kit; PD1L2_HUMAN ELISA Kit; PDCD 1 ligand 2 ELISA Kit; PDCD1 ligand 2 ELISA Kit; PDCD1L2 ELISA Kit; Pdcd1lg2 ELISA Kit; PDL 2 ELISA Kit; PDL2 ELISA Kit; Programmed cell death 1 ligand 2 ELISA Kit; Programmed death ligand 2 ELISA Kit
  • 缩写：
    PDCD1LG2
  • Uniprot No.：
    Q9BQ51
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.45 ng/ml-30 ng/ml.
  • 灵敏度：
    0.11 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human PDCD1LG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    91


    Range %

    86-97


    1:2

    Average %

    97


    Range %

    92-103


    1:4

    Average %

    101


    Range %

    97-105


    1:8

    Average %

    89


    Range %

    83-95


  • 回收率：

    The recovery of human PDCD1LG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    85-93


    EDTA plasma (n=4)

    101

    96-104


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    30

    2.788

    2.899

    2.844

    2.745


    15

    2.365

    2.456

    2.411

    2.312


    7.5

    1.545

    1.636

    1.591

    1.492


    3.75

    0.778

    0.745

    0.762

    0.663


    1.8

    0.423

    0.411

    0.417

    0.318


    0.9

    0.256

    0.265

    0.261

    0.162


    0.45

    0.155

    0.164

    0.160

    0.061


    0

    0.101

    0.097

    0.099




  • 货期：
    3-5 working days

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