首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔降钙素原(PCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PCT含量。PCT即降钙素原，在健康人群中含量极低。当机体发生细菌感染时，PCT会明显升高。其研究机制主要是通过检测它在血液中的水平，辅助医生判断是否存在细菌感染、评估感染严重程度及指导抗生素的合理使用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，常用于科研领域评估炎症程度及疾病进展；可为感染性疾病动物模型研究、免疫机制探索及药物疗效评价提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PCT
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-102 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 81-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-100 | |||||
回收率：
| The recovery of rabbit PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 86-94 | ||||
| EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.8602.7862.8232.7065002.4472.3192.3832.2662501.6431.5881.6161.4991250.8920.9070.9000.78362.50.4630.4520.4580.34131.20.3130.3210.3170.20015.60.2210.2120.2170.10000.1190.1150.117 | ||||||
货期：3-5 working days
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