首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板活化因子(PAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PAF含量。血小板活化因子（PAF）是一种具有广泛生物活性的磷脂介质。其在炎症、过敏、心血管疾病等病理过程中扮演重要角色。研究机制集中在PAF与其受体结合，激活细胞内信号通路，引发一系列生理效应，是药物研发的潜在重要靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研场景下对大鼠模型中PAF表达的动态分析，例如评估炎症性疾病动物模型的PAF释放水平、研究药物或基因干预对PAF信号通路的影响，或探索PAF在心血管、免疫相关疾病中的作用机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PAF
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 86-98 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of rat PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-92 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.6112.7102.6612.544502.0582.1572.1081.991251.4871.5061.4971.38012.50.8990.9080.9040.7876.250.4660.4570.4620.3453.120.2900.2810.2860.1691.560.1820.1870.1850.06800.1180.1150.117 | ||||||
货期：3-5 working days
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