首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血小板活化因子(PAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆、细胞裂解物样本中的PAF含量。PAF即血小板活化因子，广泛参与炎症反应、过敏反应等。其背景在于它是一种强效的内源性磷脂介质。研究机制上，主要通过与特定受体结合，激活细胞内信号通路，引发系列生理病理反应，在多种疾病发生发展中扮演重要角色。试剂盒检测范围为4.69 ng/mL-300 ng/mL，该产品可为心血管疾病机制研究、炎症反应模型构建、药物靶点筛选等科研领域提供PAF浓度的精准检测，尤其适用于体外实验模型中PAF动态变化的定量分析，帮助研究者深入探究其在细胞信号传导、免疫调控及病理过程中的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PAF
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates, cell lysates
检测范围：4.69 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 80-98 | |||||
回收率：
| The recovery of human PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.0972.0932.0951.9941501.8231.7931.8081.707751.4761.3741.4251.32437.51.0290.9861.0080.90718.750.7820.7720.7770.6769.380.4210.4010.4110.3104.690.2180.2020.2100.10900.1050.0970.101 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PAF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled PAF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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