Rabbit Monoclonal Phospho-Rad17(S656) Antibody

The Phospho-Rad17(S656) antibody is a specialized tool used to detect phosphorylation of the Rad17 protein at serine 656. a post-translational modification critical for its role in DNA damage response. Rad17. a checkpoint protein, forms part of the 9-1-1 (RAD9-HUS1-RAD1) complex-loading machinery, which is recruited to sites of DNA damage or replication stress. Phosphorylation at S656. often mediated by ATR (ataxia-telangiectasia and Rad3-related) kinase, facilitates checkpoint activation by stabilizing interactions between Rad17 and other DNA repair or signaling proteins. This modification is tightly linked to cell cycle arrest, allowing time for DNA repair before replication or mitosis proceeds. Researchers use the Phospho-Rad17(S656) antibody in studies exploring genomic instability, cancer biology, or therapeutic responses to DNA-damaging agents (e.g., chemotherapy, radiation). It is commonly applied in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess checkpoint activation status. Dysregulation of Rad17 phosphorylation has been implicated in tumorigenesis and resistance to cancer therapies, making this antibody valuable for both mechanistic studies and potential biomarker development. Proper controls (e.g., phosphatase treatment) are essential to confirm phosphorylation-specific signals.