WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 1/20-1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | 60S ribosomal protein L40; CEP52; HUBCEP52; RPL40; UBA 52; Ubiquitin 52 amino acid fusion protein; Ubiquitin 60S ribosomal protein L40; Ubiquitin carboxyl extension protein 52; Ubiquitin CEP52;;UBA52 |
WB Predicted band size | Calculated MW: 15 kDa ; Observed MW: 10 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human UBA52 |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于UBA52抗体的3篇参考文献(模拟示例,请根据实际文献调整):
1. **文献名称**: "Characterization of UBA52 as a ubiquitin-ribosomal fusion protein"
**作者**: Hochstrasser, M. et al.
**摘要**: 该研究解析了UBA52的分子结构,证实其作为泛素与核糖体蛋白L40的融合前体功能,并开发了特异性抗体用于检测其在酵母和哺乳动物细胞中的表达及定位。
2. **文献名称**: "UBA52 autoantibodies in systemic lupus erythematosus"
**作者**: Kristensen, T. et al.
**摘要**: 通过ELISA和免疫印迹实验,发现UBA52在系统性红斑狼疮(SLE)患者血清中可作为自身抗原,其抗体水平与疾病活动性相关,提示其潜在的临床诊断价值。
3. **文献名称**: "UBA52 regulates proteasome activity via ubiquitin homeostasis"
**作者**: Witke, S. et al.
**摘要**: 利用UBA52基因敲除模型和特异性抗体,揭示了UBA52通过调控游离泛素水平影响蛋白酶体功能,进而参与细胞应激反应和凋亡的分子机制。
**注意**:以上为模拟摘要,实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“UBA52 antibody”、“UBA52 ubiquitin”进行精确查找。
The UBA52 antibody is designed to detect UBA52. a ubiquitin-ribosomal fusion protein encoded by the UBA52 gene in humans. Ubiquitin, a highly conserved 76-amino-acid protein, plays a central role in the ubiquitin-proteasome system (UPS), which regulates protein degradation, DNA repair, and immune responses. UBA52 is unique as it encodes a single ubiquitin monomer fused to the ribosomal protein L40 (RPL40), distinguishing it from polyubiquitin genes (UBB, UBC) that produce tandem ubiquitin repeats. This fusion protein undergoes post-translational cleavage to release functional ubiquitin and RPL40. both critical for cellular homeostasis.
UBA52 antibodies are widely used in research to study ubiquitin dynamics, protein turnover, and ribosomal biogenesis. They help identify UBA52 expression levels in diseases like cancer, neurodegenerative disorders, and immune dysregulation, where UPS dysfunction is implicated. These antibodies are validated for techniques such as Western blotting, immunofluorescence, and immunoprecipitation, often requiring specificity checks to avoid cross-reactivity with other ubiquitin sources.
Understanding UBA52's role through such antibodies provides insights into cellular stress responses, proteostasis, and therapeutic targeting of UPS-related pathologies. Proper validation ensures accurate detection, given the ubiquitous nature of ubiquitin and its homologs.
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