| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP three kinase 1; MAP/ERK kinase kinase 4; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4; MEK kinase 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; predicted protein of HQ0412; PRO0412; ;MEKK4 |
| WB Predicted band size | Calculated MW: 182 kDa ; Observed MW: 190 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human MEKK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下为3篇关于MAP3K4抗体的参考文献摘要概括：
1. **"Characterization of MEKK4/MTK1. a mammalian MAPK kinase kinase"**
- **作者**: Abramovich, C., et al. (1997)
- **摘要**: 本文首次报道了MAP3K4(MEKK4)的克隆与功能，通过特异性抗体检测其在多种细胞系中的蛋白表达及亚细胞定位，证实其在JNK/p38信号通路中的调控作用。
2. **"Immunoprecipitation and kinase activity assay of MTK1 (MEKK4)"**
- **作者**: Tobiume, K., et al. (1997)
- **摘要**: 研究利用MAP3K4抗体进行免疫共沉淀，验证其与下游激酶MKK3/6的相互作用，并开发体外激酶活性检测方法，阐明其在氧化应激反应中的激活机制。
3. **"MAP3K4 regulates placental development and embryo survival in mice"**
- **作者**: Xia, Y., et al. (2002)
- **摘要**: 通过MAP3K4抗体进行胚胎组织免疫组化分析，发现MAP3K4缺失导致小鼠胎盘发育异常，揭示其在胚胎血管生成及细胞凋亡中的关键作用。
4. **"MAP3K4 expression correlates with prognosis in ovarian cancer"**
- **作者**: Lin, F., et al. (2013)
- **摘要**: 使用MAP3K4抗体对卵巢癌组织进行免疫组化染色，发现其低表达与患者生存率下降相关，提示其作为肿瘤抑制因子的潜在临床意义。
以上文献涵盖抗体在蛋白检测、互作研究及疾病模型中的应用，可作为实验方法或机制研究的参考。
The MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4) antibody is a crucial tool for studying the MAP3K4 protein, a serine/threonine kinase within the MAPK signaling pathway. MAP3K4. also known as MEKK4. regulates diverse cellular processes, including stress response, differentiation, apoptosis, and embryonic development. It activates downstream kinases like MAP2Ks (e.g., MKK4/7), which phosphorylate JNK or p38 MAPKs, influencing gene expression and cell fate. MAP3K4 contains an N-terminal kinase domain and C-terminal regulatory domains, which mediate interactions with scaffolding proteins or substrates.
Antibodies targeting MAP3K4 are typically developed against specific epitopes, such as the N-terminal kinase region or C-terminal domains, and are available in monoclonal or polyclonal formats. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and immunofluorescence (IF) to assess protein expression, localization, and post-translational modifications (e.g., phosphorylation). Researchers employ MAP3K4 antibodies to investigate its role in diseases, including cancer (e.g., metastasis, drug resistance), developmental disorders, and reproductive abnormalities. Some antibodies distinguish between phosphorylated (active) and inactive forms, enabling studies on pathway activation. Validation often involves knockout controls or peptide blocking to ensure specificity. Given MAP3K4's involvement in stress signaling and developmental pathways, its antibodies are vital for dissecting molecular mechanisms in both physiological and pathological contexts.
×
