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产品描述：人轴突生长诱向因子1(Ntn1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的NTN1含量。NTN1是一个靶点。它在生物学领域可能与细胞迁移、神经发育等生理过程相关。目前研究或许聚焦于其信号传导机制，解析它在疾病发生如肿瘤转移等过程中扮演的角色，旨在为开发针对性的诊断方法和治疗药物提供理论基础。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经生物学研究中轴突导向机制探索、肿瘤微环境内细胞趋化性分析，或心血管研究中血管内皮细胞迁移调控等科研场景，为体外模拟生理/病理条件下Ntn1的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：epididymis tissue protein Li 131P ELISA Kit; NET1_HUMAN ELISA Kit; Netrin 1, mouse, homolog of ELISA Kit; Netrin 1-like ELISA Kit; Netrin-1 ELISA Kit; Netrin1 ELISA Kit; Ntn 1 ELISA Kit; NTN 1L ELISA Kit; NTN1 ELISA Kit; NTN1L ELISA Kit; Unc6 ELISA Kit
缩写：NTN1
Uniprot No.：O95631
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ntn1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-101 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of human Ntn1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 100 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.7452.7502.7482.606 10002.4322.3122.3722.230 5001.8331.8271.8301.688 2501.0451.0661.0560.914 1250.6580.7030.6810.539 62.50.4010.4320.4170.275 31.250.2660.2750.2710.129 00.1410.1430.142 | ||||||
货期：3-5 working days
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