首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经营养因子4(NT-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NTF4含量。NTF4（神经生长因子-4）是一种神经营养因子，由NTF4基因编码，主要通过TrkB受体信号传导。研究发现，NTF4在神经发生中起关键作用，通过调节Stat3和PKB/Akt活性，抑制Il6和Notch信号通路，促进神经干细胞分化为神经元。试剂盒检测范围为47 pg/mL-3000 pg/mL，本试剂盒适用于神经发育、损伤修复机制研究，以及神经退行性疾病模型、药物干预实验中NT - 4的动态监测，例如脑组织、外周神经或血液样本的分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ntf4 ELISA Kit; Nt4 ELISA Kit; Ntf5 ELISA Kit; Neurotrophin-4 ELISA Kit; NT-4 ELISA Kit; Neurotrophin-5 ELISA Kit; NT-5 ELISA Kit; Neutrophic factor 4 ELISA Kit
缩写：NTF4
Uniprot No.：P34131
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-94 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of rat NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.2682.2502.2592.15015001.8451.8711.8581.7497501.4641.4921.4781.3693751.1351.1491.1421.033187.50.7040.7240.7140.605940.4380.4570.4480.339470.2870.2940.2910.18200.1080.1100.109 | ||||||
货期：3-5 working days
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