Human Neurotrophin-3,NT-3 ELISA KIT

• 产品描述：
  人神经营养因子3(NT-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的NT-3含量。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于神经生物学研究、药物作用机制探索或疾病模型构建等场景，如神经再生研究、神经保护剂筛选或神经系统疾病相关生物标志物检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
• 别名：
  HDNF ELISA Kit; MGC129711 ELISA Kit; Nerve growth factor 2 ELISA Kit; Neurotrophic factor ELISA Kit; Neurotrophin 3 precursor ELISA Kit; Neurotrophin-3 ELISA Kit; Neurotrophin3 ELISA Kit; NGF 2 ELISA Kit; NGF-2 ELISA Kit; NGF2 ELISA Kit; NT 3 ELISA Kit; NT-3 ELISA Kit; NT3 ELISA Kit; NTF 3 ELISA Kit; NTF3 ELISA Kit; NTF3_HUMAN ELISA Kit
• 缩写：
  NT-3
• Uniprot No.：
  P20783
• 种属：
  Homo sapiens (Human)
• 样本类型：
  serum, plasma, cell culture supernates
• 检测范围：
  1.25 ng/mL-80 ng/mL
• 灵敏度：
  0.31 ng/mL
• 反应时间：
  1-5h
• 样本体积：
  50-100ul
• 检测波长：
  450 nm
• 研究领域：
  Neuroscience
  
• 测定原理：
  quantitative
• 测定方法：
  Sandwich
• 精密度：

  Intra-assay Precision (Precision within an assay): CV%<8%
  Three samples of known concentration were tested twenty times on one plate to assess.
  Inter-assay Precision (Precision between assays): CV%<10%
  Three samples of known concentration were tested in twenty assays to assess.

• 线性度：

  To assess the linearity of the assay, samples were spiked with high concentrations of human NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  		Sample		Serum(n=4)
  1:1		Average %		86
  		Range %		80-92
  1:2		Average %		95
  		Range %		91-99
  1:4		Average %		95
  		Range %		92-104
  1:8		Average %		94
  		Range %		87-98

• 回收率：

  The recovery of human NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
  Sample Type		Average % Recovery		Range
  Serum (n=5)		95		89-99
  EDTA plasma (n=4)		94		90-98

• 标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• ng/ml	OD1	OD2	Average	Corrected
  80	2.181	2.081	2.131	1.999
  40	1.356	1.256	1.306	1.174
  20	0.705	0.685	0.695	0.563
  10	0.446	0.436	0.441	0.309
  5	0.270	0.260	0.265	0.133
  2.5	0.230	0.224	0.227	0.095
  1.25	0.187	0.184	0.186	0.054
  0	0.132	0.131	0.132

• 
  
• 货期：
  3-5 working days