| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERLIN2; NET32; SPFH2;;Erlin 2 |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Erlin 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Erlin-2抗体的示例参考文献(部分信息为示例性概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*Erlin-2/SPFH2 forms a membrane complex with SPFH1 to regulate cholesterol homeostasis*
**作者**：Brown, J.E., et al.
**摘要**：研究利用Erlin-2特异性抗体，通过免疫共沉淀和蛋白质组学分析，揭示了Erlin-2与SPFH1在内质网中形成复合物，调控胆固醇代谢及内质网相关降解(ERAD)的作用机制。
2. **文献名称**：*Antibody-based detection of Erlin-2 in ER stress responses*
**作者**：Wang, L., et al.
**摘要**：通过Western blot和免疫荧光技术，验证了Erlin-2抗体在细胞应激模型中的特异性，发现Erlin-2在内质网应激条件下表达上调，并参与未折叠蛋白反应(UPR)的调控。
3. **文献名称**：*Role of Erlin-2 in amyloid-β protein precursor processing: Insights from antibody-mediated knockdown*
**作者**：Garcia, M., et al.
**摘要**：利用Erlin-2抗体进行功能研究，发现Erlin-2与γ-分泌酶复合物相互作用，影响淀粉样前体蛋白(APP)的加工过程，可能与阿尔茨海默病病理相关。
4. **文献名称**：*Characterization of Erlin-2 knockout mice using isoform-specific antibodies*
**作者**：Zhang, R., et al.
**摘要**：通过特异性抗体对比野生型与敲除型小鼠组织样本，证实Erlin-2缺失导致内质网结构异常和脂质代谢紊乱，提示其生理重要性。
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**注意**：上述文献为示例性内容，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词：Erlin-2 antibody, SPFH2. ER membrane complex)。
The Erlin-2 antibody is a tool used to study Erlin-2. a protein encoded by the *SPFH2* gene in humans. Erlin-2. along with Erlin-1. forms part of the Erlin1/2 complex, which localizes to the endoplasmic reticulum (ER) membrane. This complex is implicated in ER-associated degradation (ERAD), a quality control pathway that targets misfolded proteins for proteasomal degradation. Specifically, Erlin-2 has been linked to the regulation of inositol 1.4.5-trisphosphate receptors (IP3Rs), calcium signaling, and cholesterol homeostasis.
Research highlights Erlin-2's role in binding and facilitating the degradation of activated IP3Rs, thereby modulating intracellular calcium dynamics. Dysregulation of Erlin-2 has been associated with neurological disorders, including hereditary spastic paraplegia (SPG18 subtype) and amyotrophic lateral sclerosis (ALS). Antibodies against Erlin-2 enable the detection, quantification, and localization of the protein in cellular and tissue samples, aiding investigations into its physiological and pathological functions.
These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Their development has been critical for elucidating interactions within the ERAD machinery, Erlin-2's oligomerization with Erlin-1. and its involvement in disease pathways. Ongoing studies focus on Erlin-2's potential as a therapeutic target or biomarker in neurodegenerative and metabolic disorders.
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