| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HDGF; HMG1L2;;HDGF |
| WB Predicted band size | Calculated MW: 27 kDa ; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human HDGF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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1. **"Hepatoma-derived growth factor promotes tumor progression in hepatocellular carcinoma"**
- **作者**: Nakamura H, et al.
- **摘要**: 研究揭示了HDGF在肝癌细胞中的高表达及其与患者不良预后的关联。HDGF通过激活PI3K/AKT信号通路促进肿瘤细胞增殖和侵袭，提示其作为潜在治疗靶点的价值。
2. **"HDGF enhances angiogenesis through transcriptional regulation of VEGF in lung cancer"**
- **作者**: Hu TH, et al.
- **摘要**: 该文献发现HDGF在肺癌组织中通过上调血管内皮生长因子(VEGF)的表达促进肿瘤血管生成。动物模型显示，抑制HDGF可显著减少肿瘤血管密度，延缓肿瘤生长。
3. **"Targeting HDGF with neutralizing antibody suppresses gastric cancer metastasis"**
- **作者**: Enomoto H, et al.
- **摘要**: 研究者开发了一种抗HDGF单克隆抗体，并在胃癌模型中验证其效果。结果显示，抗体通过阻断HDGF与细胞表面受体的结合，抑制肿瘤转移并延长小鼠生存期。
4. **"HDGF as a prognostic biomarker and therapeutic target in hepatocellular carcinoma"**
- **作者**: Zhang J, et al.
- **摘要**: 文章分析了HDGF在肝癌中的临床意义，发现其表达水平与肿瘤分期和患者生存率显著相关。体外实验表明，HDGF抗体可诱导肝癌细胞凋亡并抑制迁移能力。
Hepatoma-derived growth factor (HDGF) is a secreted protein initially identified in hepatocellular carcinoma cells. It belongs to the HDGF-related protein (HRP) family, characterized by a conserved N-terminal PWWP domain involved in chromatin interactions and nuclear localization. HDGF plays multifunctional roles in cell proliferation, angiogenesis, apoptosis, and tissue repair by regulating gene expression and signaling pathways. Its overexpression correlates with poor prognosis in various cancers, including liver, lung, breast, and gastrointestinal malignancies, making it a potential therapeutic target.
HDGF antibodies are essential tools for detecting HDGF expression and studying its biological functions. They enable researchers to visualize HDGF localization (nuclear or cytoplasmic) via immunohistochemistry (IHC) or immunofluorescence (IF), quantify protein levels using Western blot (WB) or ELISA, and investigate protein-protein interactions through immunoprecipitation (IP). Both polyclonal and monoclonal antibodies are available, often targeting specific regions like the N-terminal domain. Validation includes testing specificity using HDGF-knockout controls and confirming cross-reactivity across species (human, mouse, rat).
These antibodies have advanced cancer research by elucidating HDGF's role in tumor progression, metastasis, and drug resistance. Emerging studies also explore HDGF's involvement in non-cancer pathologies, such as cardiovascular diseases and pulmonary fibrosis. However, challenges remain in standardizing antibody performance across experimental conditions and ensuring consistent reproducibility in clinical applications.
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