首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经秩蛋白(NRTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NRTN含量。NRTN即神经调节素，属于胶质细胞系源性神经营养因子家族。其可通过与受体相互作用，激活下游信号通路，在神经系统发育、维持神经细胞存活与功能方面发挥重要作用。目前围绕其开展的研究，有望为神经系统疾病治疗提供新方向。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于体外研究神经退行性疾病模型中NRTN的表达动态、药物干预对神经营养因子分泌的影响，或探索神经再生相关机制等科研用途本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Neurturin ELISA Kit; Neurturin precursor ELISA Kit; NRTN ELISA Kit; NRTN_HUMAN ELISA Kit; NTN ELISA Kit; prepro-neurturin ELISA Kit
缩写：NRTN
Uniprot No.：Q99748
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NRTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 97-108 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human NRTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-97 | ||||
| EDTA plasma (n=4) | 104 | 98-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.5462.6642.6052.47040002.1962.0752.1362.00120001.4641.4891.4771.34210000.8360.8220.8290.6945000.4280.4330.4310.2962500.2830.2940.2890.1541250.1960.1900.1930.05800.1360.1340.135 | ||||||
货期：3-5 working days
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