| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | UBE4A; UBOX2; UFD2; UFD2b;;UBE4A |
| WB Predicted band size | 123 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human UBE4A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于UBE4A抗体的示例参考文献(注：由于UBE4A研究相对较少，以下内容为模拟示例，非真实文献)：
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1. **文献名称**: *"UBE4A modulates tau degradation via the ubiquitin-proteasome system in Alzheimer’s models"*
**作者**: Smith J, et al. (2020)
**摘要**: 本研究利用定制的小鼠抗UBE4A抗体，通过Western blot和免疫组化分析，揭示了UBE4A在阿尔茨海默病模型中对tau蛋白泛素化降解的调控作用，提示其可能成为治疗靶点。
2. **文献名称**: *"Development and validation of a high-specificity UBE4A antibody for cancer biomarker studies"*
**作者**: Zhang L, et al. (2018)
**摘要**: 报道了一种新型兔源UBE4A多克隆抗体的开发，通过ELISA、免疫荧光和流式细胞术验证其特异性，并发现UBE4A在结直肠癌组织中表达异常升高，可能与肿瘤进展相关。
3. **文献名称**: *"UBE4A interacts with p53: Insights from co-immunoprecipitation using a novel monoclonal antibody"*
**作者**: Tanaka K, et al. (2019)
**摘要**: 使用新开发的小鼠单克隆UBE4A抗体进行免疫共沉淀实验，发现UBE4A与p53蛋白的相互作用，暗示其在DNA损伤修复和细胞周期调控中的潜在功能。
4. **文献名称**: *"Subcellular localization of UBE4A in neuronal cells revealed by antibody-based imaging"*
**作者**: Müller R, et al. (2021)
**摘要**: 通过高分辨率免疫电镜和共聚焦显微镜技术，结合抗UBE4A抗体，明确了UBE4A在神经元突触前端的富集分布，支持其在突触蛋白稳态中的作用。
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**说明**：以上文献为模拟示例，实际研究中UBE4A相关抗体文献可能较少，建议通过PubMed或Web of Science以“UBE4A antibody”或“UBE4A ubiquitination”为关键词检索最新进展。
The UBE4A (Ubiquitination Factor E4A) antibody is a research tool designed to detect and study the UBE4A protein, a member of the ubiquitin conjugation system. UBE4A, also known as UFD2A, functions as an E3/E4 ubiquitin ligase that facilitates the elongation of ubiquitin chains on substrate proteins, targeting them for proteasomal degradation. This protein plays a critical role in protein quality control, DNA repair, and regulation of cellular processes like apoptosis and autophagy. Structurally, UBE4A contains a conserved U-box domain and tetratricopeptide repeats (TPRs), enabling interactions with molecular chaperones and co-factors such as VCP/p97.
Antibodies against UBE4A are widely used in immunoblotting (Western blot), immunoprecipitation, and immunohistochemistry to investigate its expression, localization, and interaction partners in various biological contexts. Dysregulation of UBE4A has been implicated in neurodegenerative disorders (e.g., Alzheimer’s disease) and cancers, where altered ubiquitination pathways contribute to disease pathology. Researchers utilize UBE4A antibodies to explore its role in these conditions, including its involvement in clearing misfolded proteins or modulating oncogenic signaling. Commercial UBE4A antibodies are typically validated for specificity in human, mouse, or rat samples, aiding in translational studies. Understanding UBE4A's functions through antibody-based assays provides insights into therapeutic strategies targeting ubiquitination pathways.
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