首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经毡蛋白-2(NRP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NRP2含量。NRP2即神经纤毛蛋白2，它是在血管发育、神经发育等过程中发挥作用的多功能受体。研究表明其与肿瘤血管生成、癌细胞迁移侵袭等密切相关。相关机制研究集中于其与配体结合，激活下游信号通路，进而影响细胞行为，有望成为肿瘤治疗等领域的靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可将其应用于肿瘤生物学研究，探索NRP2在血管新生、癌细胞侵袭转移中的作用机制，也可用于神经退行性疾病模型中蛋白动态变化的分析。本试剂盒兼容多种生物样本类型，支持体外实验中的基础研究需求，例如细胞因子干预实验、基因编辑模型验证或药物筛选过程中的蛋白表达监测，为分子机制研究提供稳定高效的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MGC126574 ELISA Kit; Neuropilin 2 ELISA Kit; Neuropilin-2 ELISA Kit; Neuropilin2 ELISA Kit; NP 2 ELISA Kit; NP2 ELISA Kit; NPN 2 ELISA Kit; NPN2 ELISA Kit; NRP 2 ELISA Kit; Nrp2 ELISA Kit; NRP2_HUMAN ELISA Kit; PRO2714 ELISA Kit; Receptor for VEGF165 and semaphorins class3 ELISA Kit; Vascular endothelial cell growth factor 165 receptor 2 ELISA Kit; Vascular endothelial growth factor 165 receptor 2 ELISA Kit; VEGF1265R2 ELISA Kit; VEGF165R2 ELISA Kit
缩写：NRP2
Uniprot No.：O60462
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NRP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 83-96 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-91 | |||||
回收率：
| The recovery of human NRP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 98-107 | ||||
| EDTA plasma (n=4) | 97 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5402.4322.4862.375101.8992.0441.9721.86151.2321.2761.2541.1432.50.6820.6750.6790.5681.250.3860.3740.3800.2690.6250.2710.2820.2770.1660.3120.1840.1860.1850.07400.1100.1120.111 | ||||||
货期：3-5 working days
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