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产品描述：人醌NADH脱氢酶1(NQO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NQO1含量。NQO1是一种具有重要意义的靶点。它在细胞代谢和抗氧化等方面发挥作用，与多种疾病发生发展相关。研究机制上，主要围绕其酶活性、底物作用等展开，探索它在疾病进程中的角色及影响，为相关疾病的治疗提供潜在靶点和思路。试剂盒检测范围为39 pg/mL-2500 pg/mL，支持科研人员开展细胞氧化还原调控机制、疾病相关生物标志物筛选、药物干预效果评估等研究；适用于分子生物学实验室常规检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Azoreductase ELISA Kit; Cytochrome b 5 reductase ELISA Kit; DHQU ELISA Kit; DIA 4 ELISA Kit; DIA4 ELISA Kit; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) ELISA Kit; Diaphorase (NADH/NADPH) ELISA Kit; Diaphorase 4 ELISA Kit; Dioxin inducible 1 ELISA Kit; DT diaphorase ELISA Kit; DT-diaphorase ELISA Kit; DTD ELISA Kit; Menadione reductase ELISA Kit; NAD(P)H dehydrogenase [quinone] 1 ELISA Kit; NAD(P)H dehydrogenase quinone 1 ELISA Kit; NAD(P)H menadione oxidoreductase 1 dioxin inducible ELISA Kit; NAD(P)H quinone dehydrogenase 1 ELISA Kit; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 ELISA Kit; NAD(P)H:menadione oxidoreductase 1 ELISA Kit; NAD(P)H:Quinone acceptor oxidoreductase type 1 ELISA Kit; NAD(P)H:quinone oxidoreductase 1 ELISA Kit; NAD(P)H:quinone oxireductase ELISA Kit; NMOR 1 ELISA Kit; NMOR I ELISA Kit; NMOR1 ELISA Kit; NMORI ELISA Kit; NQO 1 ELISA Kit; NQO1 ELISA Kit; NQO1_HUMAN ELISA Kit; Phylloquinone reductase ELISA Kit; QR 1 ELISA Kit; QR1 ELISA Kit; Quinone reductase 1 ELISA Kit
缩写：NQO1
Uniprot No.：P15559
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NQO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 91-94 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 84-99 | |||||
回收率：
| The recovery of human NQO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 84-95 | ||||
| EDTA plasma (n=4) | 99 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected25002.6332.5512.5922.45012502.0931.9762.0351.8936251.3981.3451.3721.2303120.7330.7470.7400.5981560.4560.4940.4750.333780.2780.2920.2850.143390.2210.2170.2190.07700.1440.1400.142 | ||||||
货期：3-5 working days
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