| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BTF4; Gtf2f2; RAP30; TF2F2; TFIIF; TFIIF beta;;GTF2F2 |
| WB Predicted band size | Calculated MW: 28 kDa ; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GTF2F2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GTF2F2抗体的3篇参考文献示例(注：内容为模拟概括，具体文献需根据实际检索调整)：
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1. **文献名称**: *GTF2F2 regulates RNA polymerase II promoter-proximal pausing and elongation in human cells*
**作者**: Smith A, et al.
**摘要**: 研究通过ChIP-seq和CRISPR技术，利用GTF2F2抗体揭示其在RNA聚合酶II启动子近端暂停及延伸中的调控作用，证明其与转录复合体相互作用影响基因表达效率。
2. **文献名称**: *Structural analysis of the transcription factor IIF complex via cryo-EM*
**作者**: Chen L, et al.
**摘要**: 采用冷冻电镜技术结合GTF2F2抗体，解析了转录因子IIF复合体的三维结构，阐明了GTF2F2亚基在稳定RNA聚合酶II结合中的关键构象变化。
3. **文献名称**: *Dysregulation of GTF2F2 in hepatocellular carcinoma: Implications for diagnostic biomarkers*
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化(使用GTF2F2抗体)和Western blot分析，发现肝癌组织中GTF2F2表达异常升高，提示其可能作为潜在诊断标志物或治疗靶点。
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建议通过PubMed或Google Scholar以“GTF2F2 antibody”为关键词检索最新文献获取具体信息。
The GTF2F2 antibody targets the General Transcription Factor IIF subunit 2 (GTF2F2), a key component of the RNA polymerase II transcription machinery. GTF2F2. also known as TFIIF, forms a heterodimer with GTF2F1 to regulate transcription initiation and elongation. This complex facilitates the recruitment of RNA polymerase II to promoters, stabilizes its binding, and promotes transition from initiation to elongation phases by interacting with other transcription factors like TFIIB and TFIIH. GTF2F2 is essential for accurate gene expression and is implicated in cellular processes requiring dynamic transcriptional regulation.
Antibodies against GTF2F2 are widely used in research to study its expression, localization, and functional interactions in normal and diseased states. They are employed in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to explore transcriptional mechanisms, DNA repair pathways, and cell cycle regulation. Dysregulation of GTF2F2 has been observed in cancers and neurodegenerative disorders, making these antibodies valuable tools for investigating transcriptional abnormalities in disease models. Additionally, they aid in characterizing protein-protein interactions within the transcription preinitiation complex, providing insights into fundamental gene regulation processes. Commercial GTF2F2 antibodies are typically validated for specificity across human, mouse, and rat samples.
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