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产品描述：人神经穿透素1(NPTX-1 )酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NPTX1含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于神经科学领域的基础研究，例如探索阿尔茨海默病、帕金森病等神经退行性疾病模型中NPTX-1的表达变化，或评估药物干预对神经递质通路的影响。其宽泛的检测范围可覆盖生理及病理状态下血清/血浆样本的浓度梯度，为体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Neuronal pentraxin I ELISA Kit; Neuronal pentraxin-1 ELISA Kit; NP 1 ELISA Kit; NP-I ELISA Kit; NP1 ELISA Kit; Nptx1 ELISA Kit; NPTX1_HUMAN ELISA Kit
缩写：NPTX1
Uniprot No.：Q15818
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NPTX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 96-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human NPTX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 92 | 86-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.6422.4432.5432.3952501.8381.7371.7881.6401251.0341.1661.1000.95262.50.6130.5970.6050.45731.20.3980.4010.4000.25215.60.2640.2550.2600.1127.80.1960.1880.1920.04400.1490.1470.148 | ||||||
货期：3-5 working days
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