| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hHR6B; HR6B; RAD6B; UBC2; UBE2B;;UBE2B |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human UBE2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于UBE2B抗体的3篇参考文献示例(注：文献为虚构示例，仅供格式参考)：
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1. **文献名称**: *UBE2B interacts with BRCA1 in DNA damage repair via ubiquitination pathways*
**作者**: Smith A, et al.
**摘要**: 本研究利用UBE2B特异性抗体进行Western blot和免疫共沉淀实验，发现UBE2B与BRCA1在DNA损伤修复中形成复合物，其泛素化活性对同源重组修复至关重要。敲低UBE2B导致细胞对辐射敏感性增加。
2. **文献名称**: *Aberrant UBE2B expression in human spermatogenic failure*
**作者**: Johnson R, et al.
**摘要**: 通过免疫组化及免疫荧光技术(使用UBE2B抗体)，作者发现不育症患者睾丸组织中UBE2B蛋白表达显著降低，且定位异常，提示UBE2B缺失可能导致精子发生障碍。
3. **文献名称**: *UBE2B promotes tumor progression by stabilizing oncogenic proteins in breast cancer*
**作者**: Lee X, et al.
**摘要**: 该研究采用UBE2B抗体进行免疫印迹和免疫组织化学分析，揭示UBE2B在乳腺癌中高表达，并通过泛素化修饰稳定致癌蛋白，促进肿瘤侵袭和转移。
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(如需真实文献，建议在PubMed等数据库搜索关键词“UBE2B antibody”或结合具体研究场景筛选。)
The UBE2B antibody is a crucial tool in studying the ubiquitin-conjugating enzyme E2 B (UBE2B), a key component of the ubiquitin-proteasome system. UBE2B, also known as RAD6B, belongs to the E2 enzyme family responsible for transferring ubiquitin to target proteins, marking them for proteasomal degradation or functional modification. It plays vital roles in DNA damage repair, chromatin remodeling, and cell cycle regulation by collaborating with E3 ligases like BRCA1. UBE2B is particularly notable for its involvement in error-prone DNA repair pathways and maintenance of genomic stability.
Antibodies targeting UBE2B enable researchers to detect and quantify its expression, localization, and interactions in various biological contexts. These tools are essential for investigating UBE2B's association with cancer progression, neurodegenerative disorders, and reproductive biology. In cancer research, UBE2B overexpression has been linked to tumorigenesis and therapy resistance, making it a potential biomarker or therapeutic target. Commercially available UBE2B antibodies are validated for applications including Western blotting, immunohistochemistry, and immunofluorescence. Proper validation remains critical due to potential cross-reactivity with homologous enzymes like RAD6A (UBE2A). Studies using these antibodies have significantly advanced understanding of post-translational regulation mechanisms and their disease implications.
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