首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠心钠肽(ANP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的NPPA含量。NPPA 即心钠肽前体 A，它主要由心房肌细胞合成和分泌，在调节血压、血容量及水盐平衡等方面发挥重要作用。研究机制上，主要围绕其信号通路展开，探讨其与心血管疾病如高血压、心衰等的关联，为相关疾病的防治提供新方向。试剂盒检测范围为7.8 pg/mL-500 pg/mL，特别适用于心血管疾病动物模型研究、心脏功能分子机制探索、药物干预效果评估等科研领域，能够满足体外实验和离体组织样本的检测需求，为心血管系统相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NppaNatriuretic peptides A ELISA Kit; Prepronatriodilatin) [Cleaved into: Atrial natriuretic factor ELISA Kit; ANF ELISA Kit; Atrial natriuretic peptide ELISA Kit; ANP); Auriculin-B; Auriculin-A; Atriopeptin-1 ELISA Kit; Atriopeptin I); Atriopeptin-2 ELISA Kit; Atriopeptin II); Atriopeptin-3 ELISA Kit; Atriopeptin III)] ELISA Kit
缩写：NPPA
Uniprot No.：P01161
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 86-96 | |||||
回收率：
| The recovery of rat ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-95 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.4942.5952.5452.4232501.7221.8231.7731.6511251.1101.1311.1210.99962.50.6150.6460.6310.50931.20.4070.3960.4020.28015.60.3070.2960.3020.1807.80.1990.1960.1980.07600.1230.1210.122 | ||||||
货期：3-5 working days
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