首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人C型钠尿肽(CNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的NPPC含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，可广泛应用于心血管疾病机制研究、骨生长相关细胞模型构建、药物干预效果评估等科研领域，尤其适用于体外实验中CNP分泌水平的动态监测，为探索其生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANFC_HUMAN ELISA Kit; C type natriuretic peptide precursor ELISA Kit; CNP ELISA Kit; CNP-53 ELISA Kit; CNP2 ELISA Kit; Natriuretic peptide C ELISA Kit; Natriuretic peptide precursor C ELISA Kit; NPPC ELISA Kit
缩写：NPPC
Uniprot No.：P23582
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 92-108 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 92-104 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 88-100 | ||||||
回收率：
| The recovery of human CNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 81-94 | |||||
| EDTA plasma (n=4) | 107 | 97-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.8242.8982.8612.68920002.5742.4632.5192.34710001.9821.9861.9841.8125001.1631.1921.1781.0062500.6780.7020.6900.5181250.5720.5610.5670.39562.50.3810.3720.3770.20500.1740.1700.172 | |||||||
货期：3-5 working days
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